🇲🇾 NPRA — PKKK/200/UP/002 & PKKK/300/UP/031

◈ Seksyen Pengujian Produk & Kosmetik · Unit Penyaringan

Lovastatin HPLC Assay & Reporting

Interactive Calculation, System Suitability & Official Report Generator (Rekod Kualiti UP/008A–G)

Standardized analysis engine for the determination of Lovastatin in traditional health supplements. Includes automated System Suitability (SST) %RSD verification, 5-point calibration regression, %w/w assay, daily dose evaluation against NPRA limits (≤1.00% w/w, ≤10.00 mg/day), and printable test report drafting.

Column Thermo ODS Hypersil 200×4.6mm 5µm
Mobile Phase ACN : 0.05% H₃PO₄ (60:40 v/v)
Flow Rate 1.8 mL/min @ 45°C
Wavelength UV/PDA 238 nm
Run Time 6.0 min (Lovastatin ~4.0 min)
LOD 0.6 µg/mL (300 mg/kg)

System Suitability Test (SST) Verification

6 replicate injections of 20 µg/mL working standard solution (SOP 200 Para 6.6)
Working Standard 20 µg/mL Peak Areas
Criteria: T < 2.0
Target: ~4.0 min
SST Evaluation Summary
Mean Peak Area: 1,247,567
Std Deviation (SD): 2,883.51
Peak Area %RSD: 0.23%
%RSD Acceptance (≤ 2.0%): PASSED ✓
Tailing Factor (< 2.0): PASSED ✓
SYSTEM SUITABILITY STATUS
VALID & ACCEPTED — PROCEED WITH SAMPLE RUN

Calibration Curve & LOD Verification

5 standard concentrations + LOD check (0.6 µg/mL / 300 mg/kg) per Borang UP/008G
Standard Level Conc (µg/mL) Peak Area
Std 1
Std 2
Std 3
Std 4
Std 5
LOD Check
Regression & Linearity Results
Regression Equation: y = 62410x + 1200
Slope (m): 62,410.00
Intercept (c): 1,200.00
Correlation Coeff (r²): 0.9999
Linearity Acceptance (r² ≥ 0.999): PASSED ✓
LOD Area Threshold: 37,500 Area Counts
CALIBRATION STATUS
LINEARITY MEETS ISO/IEC 17025 CRITERIA (r² ≥ 0.999)

Sample Assay & Total Daily Dose Calculator

Quantitation and compliance determination per SOP 200 Para 6.8 & Borang UP/008A
Sample Information & Test Parameters

Gravimetric & Chromatographic Inputs
Dissolved in 100 mL flask
From Borang UP/008B (Berat Purata)
e.g., 2 capsules taken per day
Quantitation & Compliance Evaluation
Mean Sample Peak Area: 1,382,000
Lovastatin Concentration in Flask: 22.12 µg/mL
Identification Status: DIKESAN (DETECTED)
Lovastatin Content (% w/w): 0.438 % w/w
NPRA % w/w Limit (≤ 1.00% w/w): LULUS (PASS)
Lovastatin per Capsule (mg/capsule): 1.97 mg / cap
Total Daily Dose (mg/day): 3.94 mg / day
NPRA Dose Limit (≤ 10.00 mg/day): LULUS (PASS)
KEPUTUSAN AKHIR / OVERALL VERDICT
SAMPEL MEMATUHI SPESIFIKASI NPRA (PASS)

Standard Injection Sequence Protocol

Table 3 (Procedure A: Historical/ILC IQC) and Table 4 (Procedure B: Spiking IQC)